Manuals & Protocols

The External Quality Assurance Programme for Use with FAO/IAEA Rinderpest Competitive EliSA [pdf], Interim Report, IAEA, Vienna (1998).
The sero-monitoring of rinderpest throughout Africa Phase one Proceedings of a Final Research Co-ordination Meeting of the FAO/IAEA/SIDA/OAU/IBAR/PARC Co-ordinated Research Programme organized by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture and held in Bingerville, Côte d'Ivoire, 19-23 November 1990. IAEA-TECDOC-623 [pdf].
Guidelines for Sero-monitoring of Cattle Conducted by PARC. (Prepared in Co-operation with the Animal Production and Health Division of FAO, Rome, together with the EEC and PARC Co-ordinators from Nairobi, Kenya), IAEA, Vienna (1988).
Selection and Breeding of Cattle and Buffalo in Asia: Strategies and Criteria for Improved Breeding. This publication was produced in the framework of an IAEA technical cooperation project and presents the latest trends in livestock production and cattle breeding management in Asia. It includes information on traits for dairy and beef cattle, their selection criteria, and breeding objectives, proposed systems for operating a cattle breeding and genetic improvement programme in Asia, and an overview of current and future technologies for improvement of cattle breeding. The publication is intended for livestock specialists involved in all levels of cattle breeding in Asia, but also for representatives from agricultural ministries, universities, departments of livestock and veterinary services as well as public and private veterinarians and consultants.
Guidelines for Sustainable Manure Management in Asian Livestock Production Systems. IAEA TECDOC Series No. 1582 [pdf].
Application of Radioimmunoassay in Improving the Reproductive Management of Smallholder Dairy Cattle. IAEA TECDOC Series No. 1571 [pdf].
Developing Methodologies for the Use of Polymerase Chain Reaction (PCR) in the Diagnosis and Monitoring of Trypanosomosis. IAEA TECDOC Series No. 1559 [pdf].
The Use of Non-structural Proteins of Foot and Mouth Disease Virus (FMDV) to Differentiate Between Vaccinated and Infected Animals. IAEA TECDOC Series No. 1546 [pdf].
Improving the Reproductive Management of Dairy Cattle Subjected to Artificial Insemination. IAEA TECDOC Series No. 1533 [pdf].
Development and field evaluation of animal feed supplementation packages. Proceedings of the final review meeting of an IAEA Technical Co-operation Regional AFRA Project organized by the Animal Production and Health (APH), Joint FAO/IAEA Programme and held in Cairo, Egypt, 25-29 November 2000. IAEA-TECDOC-1294, Vienna (June 2002).
Application of Radioimmunoassay in Improving the Reproductive Management of Smallholder Dairy Cattle. IAEA TECDOC Series No. 1571 [pdf].
A practical approach to microsatellite genotyping with special reference to livestock population genetics. Manual [pdf, 353 kb].
Improving Artificial Breeding of Cattle in Africa Guidelines and Recommendations. IAEA TECDOC Series No. 1437 [pdf, 937 kb].
Guidelines and Recommendations for Improving Artificial Breeding of Cattle and Buffalo in Asia.
Improving Animal Productivity by Supplementary Feeding of Multinutrient Blocks, Controlling Internal Parasites, and Enhancing Utilization of Alternate Feed Resources.
Improving Artificial Breeding of Cattle and Buffalo in Asia, Guidelines and Recommendations.
Improving Artificial Breeding of Cattle in Africa Guidelines and Recommendations. IAEA TECDOC Series No. 1437 [pdf, 937 kb].
Estimation of microbial protein supply in ruminants using urinary purine derivatives. This publication presents various models, describing the quantitative excretion of purine derivatives in urine, developed for various breeds of cattle and for sheep, goat, camel and buffalo and their use for estimation of microbial protein supply in ruminant livestock. It also describes progress made over the last decade in analytical methods for determining purine derivatives, and a unique approach for estimating microbial protein supply using spot urine samples developed under the FAO/IAEA CRP. This approach of using spot urine samples dispenses with quantitative recovery of urine, enabling its use by field and extension workers for evaluation of the nutritional status of farm animals. Future areas of research are also highlighted in the book. This book is a good source of reference for research workers, students and extension workers alike.
Quantification of Tannins in Tree and Shrub Foliage. Here is the most complete guide available to the analysis of tannins. A battery of tannin methodologies is presented in a simple, clear and easy-to-understand manner. This unique covers chemical, biological and radio isotopic tannin assays. Comprehensive step-by-step protocols are presented for each method. The protocols enable non-specialists and specialists alike to implement the methods easily in the laboratory. It is an ideal laboratory manual for research scientists, graduate students, and laboratory personnel working in the fields of animal nutrition, soil nutrient management, wild life-plant interactions, and plant breeding. Kluwer Academic Publishers, Dordrecht, The Netherlands 2003.
Guidelines and Recommendations for Improving Artificial Breeding of Cattle in Africa [pdf]. AFRA AI Manual.
Quantification of tannins in tree foliage - FAO/IAEA working manual, 2000. Manual [pdf].
Estimation of rumen microbial protein production from purine derivatives in urine. A laboratory manual for the FAO/IAEA Co-ordinated Research Programme on Development, Standardization and Validation of Nuclear Based Technologies for Measuring Microbial Protein Supply in Ruminant livestock for Improving Productivity. IAEA-TECDOC-945 [pdf], Vienna (1997).
Manual for Foot-and-Mouth Disease EliSA Kit, liquid Phase Blocking EIA, Bench Protocol Version - FMDAB 1.0. IAEA, Vienna (1996).
Report on the External Quality Control Service Exercises 1-15 for the Determination of Progesterone in Skim Milk and Plasma in Farm Animals, IAEA, Vienna (1988-1996).
Manual for Trypanosomosis - Direct Antigen EliSA Kit, Bench Protocol Version - TRP 1.3. IAEA, Vienna (1996).
The External Quality Assurance Programme for Use with FAO/IAEA Rinderpest Competitive EliSA, Interim Report, IAEA, Vienna (1995).
The External Quality Assurance Programme for Use with FAO/IAEA Brucella Indirect EliSA, Interim Report, IAEA, Vienna (1995).
Manual for Rinderpest EliSA Kit - Immunocapture Enzyme Immunoassay for Detection of Antigen to Rinderpest Virus and Peste des Petits Ruminants-Bench Protocol Version ICE 1.1. IAEA, Vienna (1995).
Manual for Leucosis Indirect EliSA Kit Bench Protocol Version - BLV 1.02, IAEA, Vienna (1995).
Improving Animal Production by Nuclear Techniques. Garcia, M., Jayasuriya, M.C.N., Perera, B.M.A.O. 1995. IAEA Yearbook B17-B32.
Animal Health: Supporting Africa's Campaign against Rinderpest. Jeggo, M., Geiger, R., Dargie, J. 1994. IAEA Bull. 36:3-48.
Recommended Procedures for the Disease and Serological Surveillance as Part of the Global Rinderpest Eradication Programme (GREP). IAEA-TECDOC-747, IAEA, Vienna (1994).
Manual for Brucellosis Indirect EliSA Kit, Bench Protocol Version - BRA 1.3, IAEA, Vienna (1994).
Manual of Nutritional Metabolite Kit Protocols, IAEA, Vienna (1994).
Manual of Self-coating Milk Progesterone RIA Kit, IAEA, Vienna (1994).
Manual for Foot & Mouth Disease Indirect Sandwich EliSA Kit, Bench Protocol Version - FMDAB 1.0, IAEA, Vienna (1994).
Manual for Babesiosis Indirect EliSA Kit, Bench Protocol Version - BBO 2.1, IAEA, Vienna (1993).
Manual for Progesterone RIA Kit, Assay Protocol, Version 2.0, IAEA, Vienna (1993).
Manual for Rinderpest EliSA Kit - Competitive Enzyme Immunoassay for Detection of Antibody to Rinderpest Virus - Bench Protocol Version RPV 1.3. IAEA, Vienna (1993).
Manual on Measurement of Methane and Nitrous Oxide Emissions from Agriculture, IAEA-TECDOC-674 (1992).
Analytical Techniques for Characterizing Ruminant Feedstuffs, IAEA, Vienna (1989).
Manual for Enzyme Immunoassay Kits for Milk and Plasma Progesterone, IAEA, Vienna (1989).
Use of Enzyme-linked Immunosorbent Assay (EliSA) in Animal Disease Diagnosis, IAEA, Vienna (1988).
Guidelines for Sero-monitoring of Cattle Conducted by PARC. (Prepared in Co-operation with the Animal Production and Health Division of FAO, Rome, together with the EEC and PARC Co-ordinators from Nairobi, Kenya), IAEA, Vienna (1988).
Predicting and Improving the Safety and Efficiency of Feeding Ruminants on Tanniniferous Tree Foliage Animal Feed Science and Technology. This special issue contains the peer reviewed papers from participants in a FAO/IAEA CRP entitled ‘Use of Nuclear and Related Techniques to Develop Simple Tannin Assays for Predicting and Improving the Safety and Efficiency of Feeding Ruminants on Tanniniferous Tree Foliage’.
Estimation of microbial protein supply in ruminants using urinary purine derivatives. This publication presents various models, describing the quantitative excretion of purine derivatives in urine, developed for various breeds of cattle and for sheep, goat, camel and buffalo and their use for estimation of microbial protein supply in ruminant livestock. It also describes progress made over the last decade in analytical methods for determining purine derivatives, and a unique approach for estimating microbial protein supply using spot urine samples developed under the FAO/IAEA CRP. This approach of using spot urine samples dispenses with quantitative recovery of urine, enabling its use by field and extension workers for evaluation of the nutritional status of farm animals. Future areas of research are also highlighted in the book. This book is a good source of reference for research workers, students and extension workers alike.
Characteristics and parameters of family poultry production in Africa, Results of a FAO/IAEA Co-ordinated Research Programme on Assessment of the effectiveness of vaccinations strategies against Newcastle disease and Gumboro disease using immunoassay-based technologies for increasing farmyard poultry production in Africa, Backhuys Publishers, Leiden (2002).
Performance indicators for rinderpest surveillance. IAEA-TECDOC-1261, IAEA, Vienna (2001) [pdf, 2556kb].
Radioimmunoassay and related techniques to improve artificial insemination programmes for cattle reared under tropical and sub-tropical conditions. Proceedings of a final Research Co-ordination Meeting organized by the Animal Production and Health (APH), Joint FAO/IAEA Programme and held in Uppsala, Sweden,  10-14 May 1999 IAEA-TECDOC-1220, IAEA, Vienna (2001) [pdf, 1660kb].
Animal Trypanosomosis: Diagnosis and Epidemiology. Results of a FAO/IAEA Co-ordinated Research Programme on the use of immunoassay methods for improved diagnosis of trypanosomosis and monitoring tsetse and trypanosomosis control programmes, Backhuys Publishers, Leiden,  ISBN 90-5782-065-X (2000).
Guidelines for the use of performance indicators in rinderpest surveillance programmes. A part of the Global Rinderpest Eradication Programme (GREP) prepared by the Animal Production and Health (APH), Joint FAO/IAEA Programme) IAEA-TECDOC-1161, IAEA, Vienna (2000) [pdf, 2556kb].
Use of immunoassay technologies for the diagnosis and control of foot-and-mouth disease in Southeast Asia. Proceedings of a final Research Co-ordination Meeting organized by the Animal Production and Health (APH), Joint FAO/IAEA Programme and held in Phnom Penh, Cambodia, 22-26 February 1999, IAEA-TECDOC-1150, IAEA, Vienna (2000) [pdf, 2156kb].
Development of feed supplementation strategies for improving the productivity of dairy cattle on smallholder farms in Africa. Proceedings of the final Research Co-ordination Meeting by the Animal Production and Health (APH), Joint FAO/IAEA Programme and held in Vienna, Austria, 7-11 September 1998, IAEA-TECDOC-1102, IAEA, Vienna (1999).
The Sero-monitoring of Rinderpest throughout Africa Phase III. Results for 1998, Internal publication IAEA, Vienna (1999) [pdf, 791kb].
Nuclear based technologies for estimating microbial protein supply in ruminant livestock. Proceedings of the second Research Co-ordination Meeting of a Co-ordinated Research Project (Phase 1) organized by the Animal Production and Health (APH), Joint FAO/IAEA Programme and held in Vienna, 24-28 August 1998, IAEA-TECDOC-1093, IAEA, Vienna (1999).
The Sero-monitoring of Rinderpest throughout Africa Phase III. Results for 1997, Internal publication IAEA, Vienna (1998) [pdf, 525kb].
Diagnosis and epidemiologyof animal diseases in Latin America. Proceedings of the final Research Co-ordination Meetings of FAO/IAEA/SIDA co-ordinated research projects on Immunoassay Methods for the Diagnosis and Epidemiology of Animal Diseases in Latin America held in Guadeloupe, Lesser Antilles, France, 13-17 June 1994 and The use of ELISA for Epidemiology and Control of Foot and Mouth Disease and Bovine Brucellosis in Latin America held in Vienna, Austria 14-18 April 1997, organized by the Animal Production and Health (APH), Joint FAO/IAEA Programme, IAEA-TECDOC-1055, IAEA, Vienna (1998).
Diagnosis and Epidemiology of Animal Diseases in Latin America. Proceedings of the Final Research Co-ordination Meetings of FAO/IAEA/SIDA Co-ordinated Research Programmes entitled "Immunoassay Methods for the Diagnosis and Epidemiology of Animal Diseases in Latin America" held in Guadeloupe, Lesser Antilles, France, 13-17 June, 1994 and "The Use of ELISA for Epidemiology and Control of Foot-and-Mouth Disease and Bovine Brucellosis in Latin America" held in Vienna, Austria, 14-18 April, 1997.
Development of supplementation strategy for milk producing animals in tropical and subtropical environments. Plenary papers presented at the final Research co-ordination meeting in Malang, Indonesia, 24-28 March, 1997, special Issue of "Preventive Veterinary Medicine" Vol.38 Nos.2-3.
Estimation of rumen microbial protein production from purine derivates in urine. A laboratory manual for the FAO/IAEA Co-ordinated Research Programme on Development, Standardization and Validation of Nuclear Based Technologies for Measuring Microbial Protein Supply in Ruminant Livestock for Improving Productivity, IAEA-TECDOC-945, [pdf] IAEA, Vienna (1997).
Application of an Immunoassay Method to Improve the Diagnosis and Control of African Trypanosomosis. IAEA-TECDOC-925, Vienna (1997).
Development of Feed Supplementation Strategies for Improving Ruminant Productivity on Small-holder Farms in Latin America through the Use of Immunoassay Techniques. IAEA-TECDOC-877, IAEA, Vienna (1996).
Recommended Procedures for the Disease and Serological Surveillance as Part of the Global Rinderpest Eradication Programme (GREP), IAEA-TECDOC-747, IAEA, Vienna (1994).
Strengthening Research on Animal Reproduction and Disease Diagnosis in Asia through the Application of Immunoassay Techniques, IAEA-TECDOC-736, IAEA, Vienna (1994).
The Sero-monitoring of Rinderpest Throughout Africa: Phase II, Results for 1993, IAEA-TECDOC-772, IAEA, Vienna (1994).
Feeding Strategies for Improving Ruminant Productivity in Areas of Fluctuating Nutrient Supply, IAEA-TECDOC-691, IAEA, Vienna (1993).
Improving the Diagnosis and Control of Trypanosomiasis and Other Vector-borne Diseases of African Livestock Using Immunoassay Methods, IAEA- TECDOC-707, IAEA, Vienna (1993).
Improving the Productivity of Indigenous African Livestock, IAEA-TECDOC-708, IAEA, Vienna (1993).
Tsetse Control, Diagnosis and Chemotherapy Using Nuclear Techniques, IAEA-TECDOC-634, IAEA, Vienna (1992).
Regional Network for Latin America on Animal Disease Diagnosis Using Immunoassay and Labelled DNA Probe Techniques, IAEA-TECDOC-657, IAEA, Vienna (1992).
The Sero-Monitoring of Rinderpest Throughout Africa: Phase I, IAEA-TECDOC-623, IAEA, Vienna (1991).
Isotope Aided Studies on Goat and Sheep Production in the Tropics. Panel Proceeding Series, Perth, Australia, 20-24 February 1989. IAEA-STI/PUB/860, Vienna (1991).
Domestic Buffalo Production in Asia. Panel Proceeding Series, Rockhampton, Australia, 20-24 February 1989. IAEA-STI/PUB/855, Vienna (1990).
Studies on the Reproductive Efficiency of Cattle Using Radioimmunoassay Techniques. Panel Proceeding Series, Vienna, 5-9 September 1988. IAEA-STI/PUB/829, Vienna (1990).
Livestock Reproduction in Latin America. Panel Proceeding Series, Bogotá, 19-23 September 1988. IAEA-STI/PUB/833, Vienna (1990).
Nuclear Techniques in the Study and Control of Parasitic Diseases of Livestock. Panel Proceeding Series, Vienna, 11-14 May 1987. IAEA-STI/PUB/792, Vienna (1988).
Isotope-aided Studies on Livestock Productivity in Mediterranean and North African Countries. Panel Proceeding Series, Rabbat, Morocco, 23-27 March 1987. IAEA-STI/PUB/778, Vienna (1988).
Isotope-aided Studies on Non-protein Nitrogen and Agro-industrial By-products Utilization by Ruminants. Panel Proceeding Series, Vienna, 24-26 March 1986. IAEA-STI/PUB/748, Vienna (1987).