Blood Processing User’s Manual and Database for Tsetse Flies

Although the blood processing procedures manual and the database were done for the tsetse rearing in Kality, Ethiopia, the procedures are standard as well as the data base and can be used in any tsetse production facility.

Tsetse flies are fed on quality-tested fresh defibrinated blood, which has been stored in a frozen condition (Wetzel and Luger 1978).

The following procedures describe the collection of animal blood in the abattoir, radiation with gamma-rays (decontamination), preservation and storage in deep freeze, quality control assurance and processing of the blood into diet for feeding tsetse flies. The procedures aim to provide nutrition at a constant quality to mass-reared fly colonies maintained over a long period for field programmes. The following is the standard procedure specifically created for the insectary at Kaliti.

Before collecting the blood, all the equipment that will be in contact with blood must be thoroughly cleaned and sterilized. During the blood collection in the abattoir, the fibrin is removed by mechanically stirring the blood or by adding anticoagulants to stop the blood from clotting. If slaughtering takes place on the floor, the blood collection equipment used is selected accordingly.

After collection, a sample of blood from the batch is checked for microbial contamination, and bioassayed for its nutritive value using female adult flies. All blood fed to flies must first be decontaminated, for example by treatment with radiation.

Blood will then be stored in frozen conditions (-20 ⁰C) until the results from the quality control tests are ready. If results show no bacterial contamination and good nutritional value, blood will be thawed and prepared to be used.

Summary
Users manual
Blood processing data base (MS Access file) [Click with right button of mouse to Save Target As in your PC]