Manuals & Protocols

Manual to Differentiate Wild Mediterranean Fruit Flies Ceratitis capitata (Wied.) from non-irradiated (Fertile) and Irradiated (Sterile) VIENNA Temperature Sensitive Lethal Strain Flies. This manual is a product of recent studies on the effect of gamma radiation on testes and ovaries of the VIENNA Mediterranean fruit fly temperature sensitive lethal (TSL) genetic sexing strains, which are being used in most Mediterranean fruit fly mass-rearing facilities in the world. It includes standardised and updated procedures to determine the fertility or sterility of adults of these strains. Consequently, this is a very useful document to support SIT based area-wide integrated pest management programmes that release VIENNA Mediterranean fruit fly TSL strains.
Guideline for packing, shipping, holding and release of sterile flies in area-wide fruit fly control programmes. This 2nd edition of the guideline is an updated version of the original FAO/IAEA guideline published by FAO in 2007. The majority of the procedures described in this guideline were initially designed specifically for the Mediterranean fruit fly Ceratitis capitata (Wied.). Nevertheless, these can be applied with some modifications to other tephritid pest species of the genera Anastrepha, Bactrocera and Dacus. The guideline is designed to be a working document, subject to periodic reviews and updates based on new developments in SIT technology. Future editions will endeavour to include more specific recommendations for other species of fruit flies as the relevant data become available.
Standard Operational Procedures to Detect and Manage Glossina pallidipes Salivary Gland Hypertrophy Virus (GpSGHV) in Tsetse Fly 'Factories' [pdf]. Vienna, Austria (2015)”.
Blood Processing User’s Manual for TseTse Fly, Version 1.0. [pdf]. Vienna, Austria (2015)”.
FAO/IAEA/USDA. Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies, Version 6.0. [pdf]. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2014)”.
The Use of Mathematics in Area-Wide Integrated Pest Management Programmes Using the Sterile Insect Technique with a Special Focus on Tephritid Fruit Flies [pdf]. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, September 2013.
Trapping Manual for Area-Wide Fruit Fly Programmes [pdf]. Insect Pest Control Section of the Joint FAO/IAEA Division, International Atomic Energy Agency. Vienna, Austria, September 2013.
The FAO/IAEA Spreadsheet for Designing and Operation of Insect Mass Rearing Facilities [pdf]. FAO Plant Production and Protection and Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, FAO, Roma, Italy (2012). ISBN 978-92-5-107315-5.
FAO/IAEA Interactive Spreadsheet for Designing and Operating Insect Mass Rearing Facilities [xls].
FAO-IAEA. Rearing codling moth for the sterile insect technique [pdf]. FAO Plant Production and Protection and Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, FAO, Roma, Italy (2010). ISBN 978-92-5-106548-8.
To Kill a Pest. The use of radiation is improving the biological control of insect pests [pdf]. IAEA Bulletin (September 2009). IAEA, Vienna.
Manual for the Use of Stable Isotopes in Entomology [pdf]. IAEA-SI. IAEA, Vienna (2009). ISBN 978-92-0-102209-7.
Model Business Plan for a Sterile Insect Production Facility. IAEA-MPB. IAEA, Vienna (2008). ISBN 978-92-0-110007-8.
FAO-IAEA. Collection of Entomological Baseline Data for Tsetse Area-Wide Integrated Pest Management Programmes [pdf]. FAO Animal and Heaalth Guidelines and Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. FAO, Roma, Italy (2008). ISBN 978-92-5-106158-9.
Cactoblastis cactorum. The biology, History, Threat, Surveillance and Control of the Cactus Moth [pdf]. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2007).
FAO/IAEA Standard Operating Procedures for Mass-Rearing Tsetse Flies [pdf]. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2006).
Designing and Implementing a Geographical Information System: A Guide for Managers of Area-Wide Pest Management Programmes [pdf]. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2006).
FAO-IAEA. Integrating the Sterile Insect Technique as a Key Component of Area-Wide Tsetse and Trypanosomiasis Intervention [pdf]. PAAT Technical and Scientific Series. FAO Agriculture and Consumer Protection. FAO, Roma, Italy (2001). ISBN 92-5-104646-8.
Identification of Peach Fruit Fly, Bactrocera zonata (Saunders), in The Eastern Mediterranean, Ian M. White, The Natural History Museum, London, UK.
Action Plan Peach Fruit Fly Bactrocera zonata (Saunders) [pdf]. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2000).
Gafchromic® dosimetry system for SIT - Standard operating procedure. Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture. IAEA, Vienna, Austria (2000).
A field guide for the Diagnosis, Treatment and Prevention of African Animal Trypanosomosis [pdf]. Food and Agriculture Organization of the United Nations. FAO, Rome, Italy (1998). ISBN 92-5-104238-1.
Laboratory Training Manual on the Use of Nuclear Techniques in Insect Research and Control - Third Edition [pdf]. IAEA Technical Reports Series No. 336. IAEA, Vienna (1992). ISBN 92-0-101792-8.
Manual for the Control of the Screwworm Fly Cochliomyia hominivorax, Coquerel [pdf]. Food and Agriculture Organization of the United Nations. FAO, Rome (1990).
Manuel de Lutte Contre la Lucilie Bouchére Cochliomyia hominivorax, Coquerel [pdf]. Food and Agriculture Organization of the United Nations. FAO, Rome (1990).
Laboratory Training Manual on the Use of Isotopes and Radiation in Entomology [pdf]. Second Edition. IAEA Technical Reports Series No. 61. STI/DOC/10/61/2, IAEA, Vienna (1977). ISBN 92-0-115177-2.
Laboratory Training Manual on the Use of Isotopes and Radiation in Entomology [pdf]. IAEA Technical Reports Series No. 61. STI/DOC/10/61, IAEA, Vienna (1966).