Radio-actively Labelled DNA Probes for Crop Improvement

Objective:

The main objective of this CRP is to establish an international network for the production and free distribution of DNA probes and other DNA resources for important crops to public research institutions in developing countries (such as rice, wheat, yam, banana, plantain, pearl millet, sorghum, cowpea, mungbean and soybean) and to facilitate detection of plant diseases caused by various pathogens such as Mycosphaerella (banana and plantain), Ascochyta (chickpea), Erisyphe graminis (barley and wheat), Pyricularia grisea (rice), Erisyphe polygoni (mungbean) and others. In so doing, it is attempting to build capacity in developing countries to use DNA technologies in plant breeding programmes for the detection and isolation of desired crop plant mutations induced by radiation.

Activities:

This CRP started in 1994. During the first Research Coordination Meeting, the participants agreed that oligonucleotide based marker techniques are the most transferable and applicable molecular methods presently available. Participants discussed the progress made in their projects. An inventory was made of the available RFLP anchor probe sets for various crops. These techniques are particularly useful as diagnostic tools, but can also be used for mapping. Technology transfer in this particular case occurs through the distribution of DNA probes and pertinent information on their effective use.. In addition, arbitrary oligonucleotide primers are available on request for mapping and fingerprinting. These can be used for more demanding techniques such as DNA Amplification Fingerprinting ( DAF), Arbitary Signature from Amplified Profiles (ASAP) and Microsatellite Primed PCR,( MP-PCR) . Specific primers for yam, chickpea, banana, plantain and phytopathogenic fungi and PCR primers for viral diagnosis can also be requested from some of participants.

Participants:

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Reports:

  • Report of First RCM, Vienna, Austria, 1995
  • Report of Second RCM, Jose, Costa Rica, 20-24 January 1997. IAEA-312.D2.RC.580.2 (1998). pp 1-83.
  • Report of Final RCM, Vienna, Austria, 6-8 September 1999. November 2001 publication. IAEA-TECDOC-1253. pp 1-79.

Project Officer:

P.J.L. Lagoda