Manuals and Guidelines


Molecular Characterization of Mutant Germplasm - A Manual. Prepared by the Prepared by the Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Plant Breeding and Genetics Laboratory, Seibersdorf, 2015.
Molecular Characterization of Mutant Germplasm - A Manual. Prepared by the Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Plant Breeding and Genetics Laboratory, Seibersdorf, 2014.
Doubled haploid production in crop plants. A Manual. Kluwer Academic Publisher, The Netherlands, 2003. ISBN 1-4020-1544-5.
Molecular Techniques in Crop Improvement. S. Mohan Jain, D.S. Brar and B.S. Ahloowalia (eds.), 2002. Kluwer Academic Publishers, The Netherlands.
Mutant Germplasm Characterization using Molecular Markers. Training Course Series No.19. IAEA, Vienna (2002).
Manual on Mutation Breeding, Second Edition, Technical Report Series No. 119, IAEA, Vienna (1977).

Protocols


DNA Concentration
- DNA concentration determination – example [xls].
- DNA gel - example [tif].
- DNA Concentration - blank template [xls].
Low-cost methods for molecular characterization of mutant plants: from tissue collection to mutation discovery [pdf].
Low-cost TILLING with pooled samples using denaturing agarose gels stained with ethidium bromide [pdf].
Low-cost DNA extraction for use in TILLING and Ecotilling assays [pdf].
Low-cost DNA extraction for recalcitrant plant species [pdf].
Low cost and non-toxic genomic DNA extraction for use in molecular marker studies [pdf].
Alternative enzymology for mismatch cleavage for TILLING and Ecotilling: extraction of enzymes from weedy plants [pdf].
Salt tolerance screening in rice using hydroponics [pdf].
Positive control kit for enzymatic mismatch cleavage using LICOR and agarose gel visualization (version 2.5) [pdf].
Positive control kit for enzymatic mismatch cleavage and agarose gel visualization (version 2.4) [pdf].
Barley Crossing Methods [pdf].
Protocol for X-ray mutagenesis of plant material: seed [pdf].