Manuals, Guidelines & Protocols

Molecular Characterization of Mutant Germplasm - A Manual
Prepared by the Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Plant Breeding and Genetics Laboratory, Seibersdorf, 2014.


The first print of this manual on selected molecular marker techniques was prepared using the hand-outs and other materials distributed to participants of the FAO/IAEA Interregional Training Course on "Mutant germplasm characterization using molecular markers". While this series of courses ended in 2007, there has been a continual demand from trainees for a codified set of standard protocols, and so the Plant Breeding and Genetics Laboratory (PBGL) has continued adapting this book by incorporating new protocols with the aim of assisting Member States in the appropriate application of molecular tools with minimal costs.

These include protocols for TILLING/Ecotilling, DNA quantification, low-cost and low toxicity DNA extraction (new in 2013), alternative enzymology for enzymatic mismatch cleavage (new in 2013), and target gene selection and primer design using whole genome annotations (new in 2013).